A male scientist with googles and in lab coat is pushing a 3M encapsulated system holder loaded wit 3M encapsulated filters

3M™ Emphaze™ AEX Hybrid Purifier

The first fiber chromatographic clarification solution to remove both small insoluble and soluble contaminants.

Reduce cell debris and deliver consistent high purity

Have small soluble contaminants such as DNA and HCP created purification challenges for your downstream process? The solution: 3M™ Emphaze™ AEX Hybrid Purifier is designed to address these challenges and also to deliver consistent, high-purity clarified process fluid and to further enhance the performance of Protein A capture column.

Excellent throughput and mAb recovery
Enhanced capture column performance
Increased capture column protection
High turbidity reduction
DNA, HCP and endotoxin reduction

Watch now: One product performing multiple actions

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One step to increase efficiency

Integrating 3M™ Emphaze™ AEX Hybrid Purifier in the clarification stage can improve product purity and yield early in the process and may reduce the total cost of ownership for the mAb purification process.

A versatile solution to increase process efficiency

3M™ Emphaze™ AEX Hybrid Purifier can be easily incorporated into clarification without changing your current process, in a scalable fashion, and can provide a more efficient process from end to end. It can also replace the second stage of depth filtration.

Product recovery can be substantially increased by reducing the sterilising membrane surface area pre-Protein A, eliminating the post-viral inactivation depth filter clarification requirement and combining polishing trains into one connected operation.

By introducing a fiber chromatographic solutions in your process, you can achieve:

Pre-Protein A
Nominal 20-40% HCP reduction pre-Protein A
Protein A
10x less residual contaminants on Protein A column
Post-Protein A
10x lower HCP

1,000x DNA reduction

Removal of problematic host cell proteins (HCPs) in downsteam mAb purification

Identification and Tracking of Problematic Host Cell Proteins when using 3M™ Emphaze™ AEX Hybrid Purifier in mAb Manufacturing Processes

Implementation of 3M™ Emphaze™ AEX Hybrid Purifier during mAb clarification process can substantially enhance purification performance of the Protein A column by significantly reducing DNA and HCP load. In addition, clarification with Emphaze AEX hybrid purifier in conjunction with Protein A chromatography may reduce HCPs that are known to persist through the polishing train. Integration of Emphaze AEX hybrid purifier in the clarification stage may simplify mAb purification by reducing multiple types of impurities in a single process step, leading to better process economics.

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Endotoxin removal by 3M™ Emphaze™ AEX Hybrid Purifier

Read application data for endotoxin removal from protein solutions using 3M™ Emphaze™ AEX Hybrid Purifier - A robust and simple solution to the challenging problem of endotoxin removal to help accelerate the preclinical development of biopharmaceutical drug candidates.

3M™ Emphaze™ AEX Hybrid Purifier is ideal for endotoxin removal applications in a laboratory setting, especially for high throughput processing of protein samples for screening during pre-clinical and early-stage clinical testing. The high capacity of 3M™ Emphaze™ AEX Hybrid Purifier allows for high protein loads, and scalable products allow for easy transition to pilot or production scale processes.

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A male scientist with googles and in lab coat is wheeling the 3M encapsulated system holder loaded with 3M encapsulated filters

3M™ Emphaze™ AEX Hybrid Purifier

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Which technology is right for your process?

3M™ Harvest RC Chromatographic Clarifier

Technology Platforms

Quaternary amine (Q) functionalized polypropylene nonwoven
0.2 μm polyether sulfone (PES) membrane

Mechanism

AEX

Materials

Synthetic

Feed Stream

Direct harvest

Scalability

Linear scalability from lab to multi-capsule commercial production

Applications

Direct harvest in a single stage
Replacement of primary, secondary and guard filters

Features

High mAb product recovery (Capsules: >95%; Conical Tube and Well Plate >90%)
Consistent cell loading capacity
Turbidity reduction (<15NTU)
DNA reduction (<500 ppb)
Cell shear minimization
0.1 & 0.2 μm sterile filter protection

More product details: 3M™ Harvest RC Chromatographic Clarifier