Infographic showing that a 3-step harvest and clarification step is compressed into a single stage process by using 3M™ Harvest RC Chromatographic Clarifier

3M™ Harvest RC Chromatographic Clarifier

The next-generation solution in harvest and clarification to increase mAb yields

Rising cell densities and increasing levels of soluble and insoluble contaminants of cell cultures has been an ongoing challenge for the bioprocessing industry. If you are still using centrifuge or multi-stage depth filtration, fiber chromatographic clarification may be the answer to meet current and future needs. ​

3M™ Harvest RC Chromatographic Clarifier is a next-generation solution in harvest and clarification technology. It is designed as an efficient option to increase mAb yields while streamlining the clarification process by replacing the centrifuge and/or depth filtration process steps.

  • expansion icon
    Scalability from bench to commercial production​
  • Upward arrow under a magnifier icon
    Predictable performance
  • Recovery icon
    High product recovery
  • Elimination icon
    Whole cell removal and minimize cell sheer
  • Streamline icon
    Streamlined process and smaller footprint

Reimagine your harvest process with simplified and streamlined production

Infographic showing that a 3-step harvest and clarification step is compressed into a single stage process by using 3M™ Harvest RC Chromatographic Clarifier

AEX fiber chromatographic technology enables further compression of the process to allow for cost effective manufacturing solutions. ​

  • Replaces primary and secondary depth filters and guard membranes
  • Removes cells, cell debris, soluble and insoluble contaminates in one step

Robust scalability with predictable performance across scales

Fibrous chromatographic clarification assures scalable performance from discovery to manufacturing which allows for faster speed to clinic and market. Performance is consistent from laboratory capsules (BC4 and BC25), to scale-up capsules (BC340 and BC1020), to production capsules (BC2300 and BC16000).

  • Bar chart showing the normalised throughput by various scales of  3M™ Harvest RC Chromatographic Clarifier
  • Bar chart showing the mAb product recovery in % by various scales of  3M™ Harvest RC Chromatographic Clarifier

Biopharmaceutical industry insights

  • Cover of 3M™ Harvest RC Chromatographic Clarifier Beta Testing Series
    See what the industry says about 3M™ Harvest RC Chromatographic Clarifier

    During a Frost & Sullivan Virtual Think Tank Early Access Series, respondents made it evident that they believe adopting 3M™ Harvest RC Chromatographic Clarifier in commercial manufacturing will eliminate key challenges in the harvest process. The multiple benefits of this single-stage, single-use chromatographic clarification solution has driven its industry adoption.

    Read more of Frost & Sullivan's findings

  • Cover of Single-stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein harvest
    Read our recent co-publication with Ambrx

    The use of the 3M™Harvest RC Chromatographic Clarifier, a single-stage chromatographic clarification product, is identified as an alternative strategy to a three-stage filtration train. Using bioprocess cost modeling, the overall cost of the clarification process was reduced by 17%–30% through process compression, depending on bioreactor scale. The benefits of the single-stage harvest process extended through the downstream process, resulting in a 25% relative increase in cumulative yield with comparative impurity clearance.

    Read more


A collection of various sizes of 3M™ Harvest RC Chromatographic Clarifier
3M™ Harvest RC Chromatographic Clarifier

The next-generation solution of harvest and clarification technology to increase mAb yields while streamlining the clarification process.

VIEW PRODUCT DETAILS

Which technology is right for your process?

  • 3M™ Harvest RC Chromatographic Clarifier

    Technology Platforms
    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • 0.2 μm polyether sulfone (PES) membrane
    Mechanism
    AEX
    Materials
    Synthetic
    Feed Stream
    Direct harvest
    Scalability
    Linear scalability from lab to multi-capsule commercial production
    Applications
    • Direct harvest in a single stage
    • Replacement of primary, secondary and guard filters
    Features
    • High mAb product recovery (Capsules: >95%; Conical Tube and Well Plate >90%)
    • Consistent cell loading capacity
    • Turbidity reduction (<15NTU)
    • DNA reduction (<500 ppb)
    • Cell shear minimization
    • 0.1 & 0.2 μm sterile filter protection
    More product details: 3M™ Harvest RC Chromatographic Clarifier
  • Technology Platforms
    • Quaternary amine (Q) functionalized polypropylene nonwoven
    • Highly asymmetric 0.2 μm polyamide membrane
    Mechanism
    AEX
    Materials
    Synthetic
    Feed Stream
    Post depth filter, post centrifuge
    Scalability
    Linear scalability from lab to multi-capsule commercial production
    Applications
    • Replacement of secondary depth filters in harvest and clarification
    • Guard filter to Protein A
    • Bioburden control
    Features
    • Nominal 20-40% HCP and greater than 4 logs DNA reduction
    • Consistent output turbidity (<5NTU)
    • Typically, ≥ 4 LRV endotoxin reduction at low conductivity and neutral pH depending on buffer
    More product details: 3M™ Emphaze™ AEX Hybrid Purifier

Want to simplify your process?

Innovation in cell culture production has created additional complexities for the clarification process. 3M offers single-stage and single-use solutions to help simplify your process and increase manufacturing efficiency.

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